Blar i forfatter "Knudsen, Steen Wilhelm"

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    • Citizens in the Lab: Performance and Validation of eDNA Results 

      Tøttrup, Anders P.; Svenningsen, Lea; Rytter, Maria; Lillemark, Marie Rathcke; Møller, Peter Daniel Rask; Knudsen, Steen Wilhelm (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-12-16)
      Citizen Science has traditionally been applied in biodiversity monitoring, as the approach holds the potential for conducting large-scale data collections. However, involving citizens in more than data collection is still in its infancy. In this paper, we present the results of an ongoing citizen science project that expands the partnership between citizens and researchers by involving citizens in ...

    • Deep-sea sponge derived environmental DNA analysis reveals demersal fish biodiversity of a remote Arctic ecosystem 

      Brodnicke, Ole Bjørn; Meyer, Heidi Kristina; Busch, Kathrin; Xavier, Joana R.; Knudsen, Steen Wilhelm; Møller, Peter Daniel Rask; Hentschel, Ute Humeida; Sweet, Michael John (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-08-18)
      The deep-sea is vast, remote, and largely underexplored. However, methodological advances in environmental DNA (eDNA) surveys could aid in the exploration efforts, such as using sponges as natural eDNA filters for studying fish biodiversity. In this study, we analyzed the eDNA from 116 sponge tissue samples and compared these to 18 water eDNA samples and visual surveys obtained on an Arctic seamount. ...

    • Detection of environmental DNA from amphibians in Northern Europe applied in citizen science 

      Knudsen, Steen Wilhelm; Hesselsøe, Martin; Rytter, Maria; Lillemark, Marie Rathcke; Tøttrup, Anders P.; Rahbek, Carsten; Sheard, Julie Koch; Thomsen, Philip Francis; Agersnap, Sune; Mortensen, Peter B.; Møller, Peter Rask (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-08-25)
      Many species of amphibians in Northern Europe are threatened and the local distributions are rarely described in detail. Application of modern molecular methods provides an important supplementary tool for monitoring the distribution and diversity of amphibians. For this purpose, we designed, tested, validated, and optimized 14 species-specific assays on genomic DNA extracted from tissue samples to ...

    • Distinct latitudinal community patterns of Arctic marine vertebrates along the East Greenlandic coast detected by environmental DNA 

      Jensen, Mads Reinholdt; Høgslund, Signe; Knudsen, Steen Wilhelm; Nielsen, Julius; Møller, Peter Daniel Rask; Rysgaard, Søren; Thomsen, Philip Francis (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-12-20)
      Aim: Greenland is one of the places on Earth where the effects of climate change are most evident. The retreat of sea ice has made East Greenland more accessible for longer periods during the year. East Greenland fjords have been notoriously difficult to study due to their remoteness, dense sea ice conditions and lack of infrastructure. As a result, biological monitoring across latitudinal gradients ...

    • Holistic monitoring of freshwater and terrestrial vertebrates by camera trapping and environmental DNA 

      Holm, Anne Marie Rubæk; Knudsen, Steen Wilhelm; Månsson, Malene; Pedersen, Ditte Elmgreen; Nordfoss, Pauli Holm; Johansson, Daniel Klingberg; Gramsbergen, Marthe; Havmøller, Rasmus Worsøe; Sigsgaard, Eva Egelyng; Thomsen, Philip Francis; Olsen, Morten Tange; Møller, Peter Daniel Rask (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-10-20)
      The anthropogenic impact on the world's ecosystems is severe and the need for non-invasive, cost-effective tools for monitoring and understanding those impacts are therefore urgent. Here, we combine two such methods in a comprehensive multi-year study; camera trapping (CT) and analysis of environmental DNA (eDNA), in river marginal zones of a temperate, wetland Nature Park in Denmark. CT was ...

    • Monitoring of environmental DNA from nonindigenous species of algae, dinoflagellates and animals in the North East Atlantic 

      Knudsen, Steen Wilhelm; Hesselsøe, Martin; Thaulow, Jens; Agersnap, Sune; Hansen, Brian Klitgaard; Jacobsen, Magnus Wulff; Bekkevold, Dorte; Jensen, Søren K.S.; Møller, Peter Daniel Rask; Andersen, Jesper Harbo (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-01-14)
      Monitoring the distribution of marine non-indigenous species is a challenging task. To support this monitoring, we developed and validated the specificity of 12 primer-probe assays for detection of environmental DNA (eDNA) from marine species all non-indigenous to Europe. The species include sturgeons, a Pacific red algae, oyster thief, a freshwater hydroid from the Black Sea, Chinese mitten crab, ...

    • A National Scale “BioBlitz” Using Citizen Science and eDNA Metabarcoding for Monitoring Coastal Marine Fish 

      Agersnap, Sune; Sigsgaard, Eva Egelyng; Jensen, Mads Reinholdt; Ávila, Marcelo De Paula; Carl, Henrik; Møller, Peter Rask; Krøs, Simon Leed; Knudsen, Steen Wilhelm; Wisz, Mary S.; Thomsen, Philip Francis (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-03-28)
      Marine biodiversity is threatened by human activities. To understand the changes happening in aquatic ecosystems and to inform management, detailed, synoptic monitoring of biodiversity across large spatial extents is needed. Such monitoring is challenging due to the time, cost, and specialized skills that this typically requires. In an unprecedented study, we combined citizen science with eDNA ...

    • Short-term temporal variation of coastal marine eDNA 

      Jensen, Mads Reinholdt; Sigsgaard, Eva Egelyng; Ávila, Marcelo De Paula; Agersnap, Sune; Brenner-Larsen, William; Sengupta, Mita Eva; Xing, Yingchun; Krag, Marcus Anders; Knudsen, Steen Wilhelm; Carl, Henrik; Møller, Peter Daniel Rask; Thomsen, Philip Francis (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-02-23)
      Temporal variation in eDNA signals is increasingly explored for understanding community ecology in aquatic habitats. Seasonal changes have been addressed using eDNA sampling, but very little is known regarding short-term temporal variation that spans hours to days. To address this, we filtered marine water samples from a single coastal site in Denmark every hour for 32 h. We used metabarcoding to ...